Tesis doctoral de Nuira Jorba Grifols
The influenza virus polymerase complex is a heterotrimer formed by the pb1, pb2 and pa subunits and is required for virus transcription and replication in the nucleus of infected cells. In this doctoral thesis we present the characterization of the complexes formed intracellularly by the influenza polymerase in human cells. The three subunits of the polymerase were co-expressed in human cells and the intracellular complexes were purified by the tap approach, which involves igg-sepharose and calmodulin-agarose chromatography, under very mild conditions.
Datos académicos de la tesis doctoral «Complejos intracelulares formados por la rna polimerasa del virus de la gripe: oligomerización, factores asociados y complementación génica.«
- Título de la tesis: Complejos intracelulares formados por la rna polimerasa del virus de la gripe: oligomerización, factores asociados y complementación génica.
- Autor: Nuira Jorba Grifols
- Universidad: Autónoma de Madrid
- Fecha de lectura de la tesis: 27/05/2009
Dirección y tribunal
- Director de la tesis
- Juan Ortin Monton
- Tribunal
- Presidente del tribunal: esteban Domingo solans
- Juan Antonio Garcia alvarez (vocal)
- susana De la luna gargantilla (vocal)
- Francisco Parra fernandez (vocal)