Tesis doctoral de Iranzu Lamberto Pérez
Characterization of the cell death process induced by bisacylimidoselenocarbamate derivatives bisacylimidoselenocarbamate derivatives (bsc) are potent anticancer agents with a strong cytotoxic activity against different types of tumour cells. Based in phosphatidylserine exposure on the cell membranes we show that bsc treatment resulted in enhanced cell death in leukaemia ccrf-cem cells. Dna fragmentation detection in breast adenocarcinoma mcf-7 cells showed that bsc triggered cell death is concentration and time dependent. We also show that two of these compounds, bsc 3g and 3n, cause cell-cycle arrest in the late g2/m in mcf-7 cells. Consistent with this, a reduction in cdk1 and cdk2 expression with no change in cyclin a and b1 was observed in this cell line. Activation of caspase-2 was also detected. However, the involvement of the caspase-dependent pathway in the process of cell death induced by either bsc 3g or 3n is discarded since cell death could not be prevented by pretreatment with the pancaspase inhibitor z-vad-fmk. Moreover, since reduced levels of p21cip and chk2 proteins but no change in p53 levels could be detected in mcf-7 cells after bsc 3g or 3n treatment our results suggest that bsc treated cells die from lethal mitosis.
Datos académicos de la tesis doctoral «Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato«
- Título de la tesis: Caracterización del proceso de muerte celular inducido por los derivados bisacilimidoselenocarbamato
- Autor: Iranzu Lamberto Pérez
- Universidad: Navarra
- Fecha de lectura de la tesis: 24/10/2012
Dirección y tribunal
- Director de la tesis
- Juan Antonio Palop Cubillo
- Tribunal
- Presidente del tribunal: María Luisa Campo guinea
- almudena Porras gallo (vocal)
- víctor Martínez merino (vocal)
- María Jesús Lopez zabalza (vocal)