Tesis doctoral de Daniel Casado González
Volatile compounds from apple and walnut trees were collected in the field from attached branches, bearing leaves and fruits, enclosed in plastic bags in the morning and at dusk, in different periods of the season. Collections were analyzed by gas chromatography-mass spectrometry (gc-ms), and gas chromatography-electroantennodetection (gc-ead) using antennae of male cydia pomonella. Forty four compounds in apple and 90 in walnut were detected by gc-ms. Emissions of both plant species widely differed both qualitatively and quantitatively. Apple emissions were dominated by aliphatic compounds, whereas walnut ones by terpenes. Diel and seasonal variations were found in emissions of both plant species. Gc-ead revealed activity for 5 compounds in apple collections and 10 in walnut ones. Further electroantennographic (eag) analyses with males and females revealed important eag-activity for many other volatiles emitted by apple. In these analyses male responses were equal to or higher than those of female for all compounds, except for beta-myrcene. Amongst the ead-active compounds in the gc-ead analyses, hexyl butanoate was apple-specific, 3 compounds were walnut-specific (alloocimene, pinocarrvone, and caryophyllene oxide), and the remaining were shared by both plant species. 2-cyclopentylcyclopentanone, a compound emitted by the plastic bags, also elicited strong eag responses in antennae of both sexes. ethyl (e,z)-2,4-decadienoate (pear ester), and (e)-beta-farnesene were fully attractive for c. Pomonella in field trapping. However, they did not elicit source contacts in wind tunnel, suggesting that other sensory cues are involved in their field attractiveness. Pre-exposure to the sex pheromone, (e,e)-8,10-dodecadien-1-ol (codlemone), decreased male upwind flight to itself in wind tunnel, but increased pear ester attractiveness; and had no effect on females. Similarly, trap captures with pear ester were found to increase under mating disruption. Pear ester acted as a
Datos académicos de la tesis doctoral «Cydia pomonella (l.) behavior and responses to host volatiles«
- Título de la tesis: Cydia pomonella (l.) behavior and responses to host volatiles
- Autor: Daniel Casado González
- Universidad: Lleida
- Fecha de lectura de la tesis: 31/05/2007
Dirección y tribunal
- Director de la tesis
- Magi Riba Viladot
- Tribunal
- Presidente del tribunal: angel Guerrero perez
- fabio Molinari (vocal)
- matilde Eizaguirre altuna (vocal)
- María José Sarasua saucedo (vocal)