Tesis doctoral de María Menéndez Carreño
Cholesterol oxidation products (cops), which can be absorbed from the diet, have been well documented to exert adverse biological effects. Phytosterols can be also oxidized, giving rise to phytosterol oxidation products (pops) with several cytotoxic effects, although probably less severe than those promoted by cops. at first, the aim of this study was to develop and validate a method to detect, identify and quantify the presence of sterol oxidation products (sops) in different matrices by using a suitable gc-ms method. Cops were used as model substances assuming a similar behaviour to pops. cops have been associated with the development of atherosclerosis and cardiovascular disease (cvd). In this study, it was demonstrated that the formation of serum cops is partially inhibited by the dietary antioxidants (vitamin c and selenium) intake in rats fed on high fat diets. It has been also found that cops can be considered as efficient biomarkers to follow-up the development of cvd. recently, several phytosterol-enriched foods have appeared on the market as they have beneficial effects decreasing ldl serum levels. One of the objectives of this work was to determine how culinary heating treatments affected on these products. It can be concluded that drastic heating conditions affect the stability of sterols although the formation of sops commonly measured do not quantitatively account for all sterols losses observed during heating. Polar oxidation products, traditionally identified from sterols oxidations, are not the only degradation products formed by thermo-oxidation as non-polar and mid-polar oxidation monomeric products are also formed.
Datos académicos de la tesis doctoral «Productos de oxidación de esteroles en suero y alimentos enriquecidos: determinación, formación e implicaciones es procesos oxidativos«
- Título de la tesis: Productos de oxidación de esteroles en suero y alimentos enriquecidos: determinación, formación e implicaciones es procesos oxidativos
- Autor: María Menéndez Carreño
- Universidad: Navarra
- Fecha de lectura de la tesis: 18/09/2009
Dirección y tribunal
- Director de la tesis
- María Iciar Astiasaran Anchia
- Tribunal
- Presidente del tribunal: José alfredo Martinez hernandez
- hans-gerd Janssen (vocal)
- anna-maija Lampi (vocal)
- Ana María Troncoso gonzález (vocal)