Tesis doctoral de Sebastian Boltaña Harms
The innate immune response is based on activation the restricted number of genotypic encoded receptors, as pathogen recognition receptors (prrs). Prrs can be soluble proteins such as plasmatic pgrps or cell membrane-anchored tlrs able to recognize pathogens or their pathogen-associated molecular patterns (pamps). Pamp-prr interaction results in the activation of target genes and promotes the transduction of pro- and inflammatory mediators. The main goal of this dissertation was to characterise the responses of rainbow trout, oncorhynchus mykiss, and gilthead seabream, sparus aurata, macrophages treated with different pamps and to explore subsequent changes in the expression of immune related genes or global shifts in the macrophage transcriptome. A particular goal of this study was to register changes in macrophages activated toward an inflammatory phenotype after treatments with crude gram negative bacterial lipopolysaccharide (lps) preparations, highlighting that peptidoglycan (pgn) is contaminant within crude lps and is able to induce the mrna expression of il-1íY and il-6 and release inflammatory products as prostaglandins. Microarray analyses were made to describe concentration and time-dependent transcriptional modulations both in trout and seabream macrophages treated with pgn or lps. In the case of sea bream, a specific oligonucleotide microarray was designed and validated for these studies. Results reveal up-regulation of specific mrna transcripts that are tightly related to prostaglandin synthesis and tlr signalling pathways. Thus pgn recognition in fish is a result of recognition mechanisms including non-tlr prrs such as pgrps and nods that are conserved in the vertebrate innate immune response.
Datos académicos de la tesis doctoral «Molecular characterisation of the underlying mechanisms of pathogen-associated molecular pattern (pamp) recognition in fish«
- Título de la tesis: Molecular characterisation of the underlying mechanisms of pathogen-associated molecular pattern (pamp) recognition in fish
- Autor: Sebastian Boltaña Harms
- Universidad: Autónoma de barcelona
- Fecha de lectura de la tesis: 06/06/2011
Dirección y tribunal
- Director de la tesis
- LLuis Tort Bardolet
- Tribunal
- Presidente del tribunal: Antonio Figueras huerta
- iñaki álvarez pérez (vocal)
- (vocal)
- (vocal)