Functional genetics of suberin: the role of cyp86a33 and stkcs6 in potato tuber periderm

Tesis doctoral de Olga Serra Figueras

The functional characterization of two genes in the periderm, the ¿-hydroxylase cyp86a33 -candidate for the functionalization of the ¿-terminal carbon of suberin aliphatic compounds- and the putative ketoacyl-coa synthase stkcs6 -candidate for the elongation of vlcfa and derivatives of suberin and waxes of periderm- is performed by rna interference-mediated silencing in potato the cyp86a33 deficiency leads to a great reduction of the main suberin monomers, the c18:1 ¿-hydroxyacid and ¿,¿-diacid, together with an overall decrease of the suberin total amount by 60%. The deficiency in these ¿-oxidized fatty acids alters the typical suberin lamellar structure as well as the periderm water barrier function. stkcs6 deficiency leads to a decrease of suberin and wax compounds with chain-length c28 and higher and an increase of those with chain-length c26 and lower. This deficiency suggests that the aliphatics chain-length can contribute to the sealing properties of periderm.

 

Datos académicos de la tesis doctoral «Functional genetics of suberin: the role of cyp86a33 and stkcs6 in potato tuber periderm«

  • Título de la tesis:  Functional genetics of suberin: the role of cyp86a33 and stkcs6 in potato tuber periderm
  • Autor:  Olga Serra Figueras
  • Universidad:  Girona
  • Fecha de lectura de la tesis:  12/12/2008

 

Dirección y tribunal

  • Director de la tesis
    • María Luisa Molinas De Ferrer
  • Tribunal
    • Presidente del tribunal: salomé Prat monguió
    • jacqueline Grima-pettenatti (vocal)
    • thomas james Roscoe (vocal)
    • rochus Franke (vocal)

 

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