Tesis doctoral de Sonia Villapol Salgado
Although it is well established that apoptotic mechanisms play a central role in neuronal death after different types of cns damage, little is known about the activation of apoptotic pathways in glial cells which have been postulated to be important for the termination of the glial response. It is remarkable that the classically defined main executioner of programmed cell death, caspase-3, has recently been attributed a non-apoptotic role, participating in cellular events including cell proliferation, cell cycle regulation, or cellular differentiation. Accordingly, the general aim of this thesis was to analyze the putative role of the classic apoptotic and anti-apoptotic pathways after acute excitotoxic damage to the immature rat brain. For this purpose, a well-characterized in vivo model of excitotoxicity was used, where damage was induced by the intracortical injection of n-methyl-d-asparate (nmda) in postnatal day 9 rats. Cell death, apoptotic and anti-apoptotic proteins were analyzed at different survival times after the lesion by enzymatic assays and double immunofluorescence for confocal microscope analysis.
Datos académicos de la tesis doctoral «Apoptotic and antiapoptotic mechanisms in neurons and glial cells after damage to the immature brain«
- Título de la tesis: Apoptotic and antiapoptotic mechanisms in neurons and glial cells after damage to the immature brain
- Autor: Sonia Villapol Salgado
- Universidad: Autónoma de barcelona
- Fecha de lectura de la tesis: 19/10/2007
Dirección y tribunal
- Director de la tesis
- Laia Acarin Pérez
- Tribunal
- Presidente del tribunal: Jose Rodriguez Alvarez
- Cristina Munoz-pinedo (vocal)
- Valérie Petegnief (vocal)
- Ann Chowen Julie (vocal)