Tesis doctoral de Carme Costa Riu
The extracellular matrix (ecm) of the central nervous system (cns) is found dispersed in the neuropil or forming aggregates around the neurons called perineuronal nets (pnns). The ecm mainly contains chondroitin sulphate proteoglycans (cspg), hyaluronic acid (ha) and tenascin-r. Heparan sulphate proteoglycans (hspg) can also be secreted in the ecm or be part of the cell membrane. Aquaporins (aqp) are a family of transmembrane proteins that act as water selective channels. Aqp1 and aqp4 are widely expressed in the cns where they play several roles. Nevertheless the importance of these elements, their distribution in the cns of mice is only partially known. the histochemical distribution of pnns, aggrecan, ha, hspgs and aqp4 were semiquantitatively evaluated in the whole cns of mice. The results showed that aggrecan, ha and hspgs have a perineuronal distribution, and aqp4 has an heterogeneous distribution throughout the neuropil of the cns. An inverse correlation between aqp4 and ecm components was observed, suggesting a complementary role in the maintenance of water homeostasis. A common location for aqp4 and hspgs was also observed in cns neuropil. ecm and aqps were studied in bovine spongiform encephalopathy (bse) and scrapie. Both diseases belong to the group of animal transmissible spongiform encephalopathies (tse) or prion diseases. They are characterized by the accumulation of resistant prion protein (prpres) in the cns, neuronal loss, spongiform degeneration and glial cell proliferation. the ecm was studied by immunohistochemistry in the transgenic mice botg110, overexpresing bovine prpc, intracranially infected with cattle bse; and in c57bl/6 mice intraperitoneally infected with rml scrapie strain. Both of them showed dramatical ecm disturbances at latest stage of both diseases. aqp1 and aqp4 were studied in botg110 mice by immunohistochemistry, and in bse field cases by immunohistochemistry and western blot. Both aqps were overexpressed in the membrane o
Datos académicos de la tesis doctoral «Study of extracellular matrix and water channels in bovine spongiform encephalopaty.«
- Título de la tesis: Study of extracellular matrix and water channels in bovine spongiform encephalopaty.
- Autor: Carme Costa Riu
- Universidad: Autónoma de barcelona
- Fecha de lectura de la tesis: 29/10/2007
Dirección y tribunal
- Director de la tesis
- Marti Pumarola Batlle
- Tribunal
- Presidente del tribunal: Juan Jose Badiola Diez
- Sílvia Sisó Lloch (vocal)
- Juan a María Flores Landeira (vocal)
- Carmen Espejo Ruiz (vocal)