Tesis doctoral de Juliana De Souza RebouÁ§as
Peanut allergy is responsible by the most severe allergic reactions by anaphylaxis induction. However, there is currently no immunotherapy against this disease. The aim of this work was to evaluate the potential application of poly(anhydride) nanoparticles (np) in peanut immunotherapy. Thus, poly(anhydride) np loaded with raw or roasted peanut proteins were developed by the solvent displacement method, with some modifications. Then, the np formulations were dried by lyophilization or spray-drying and their physico-chemical characteristics were determined. The capacity of these polymeric systems was evaluated after intradermal or oral immunization of c57bl/6 mice with a single dose of the different np formulations. The adjuvant effect of all np formulations was demonstrated by a stronger and balanced th1 (igg2a) and th2 (igg1) specific antibody responses. Furthermore, animals immunized with spray-dried-np loaded with peanut proteins (raw or roasted) showed higher levels of igg2a than lyophilized-np, associated with a lower ige induction. In addition, we achieved a significant decrease of th2 cytokines (il-4, il-5 and il-6) and an enhancement of a th1 cytokine (inf-í£) secretion. Thus, immunization with poly(anhydride) np leads to a robust pro-th1 immune response, characterized by a high th1/th2 ratio. In conclusion, poly(anhydride) nanoparticles promise as a novel approach to immunotherapy against peanut allergy
Datos académicos de la tesis doctoral «Nanopartículas de poli(anhídrido)maléico como adyuvante en inmunoterapia alergénica«
- Título de la tesis: Nanopartículas de poli(anhídrido)maléico como adyuvante en inmunoterapia alergénica
- Autor: Juliana De Souza RebouÁ§as
- Universidad: Navarra
- Fecha de lectura de la tesis: 26/10/2012
Dirección y tribunal
- Director de la tesis
- Carlos Gamazo De La Rasilla
- Tribunal
- Presidente del tribunal: Juan manuel Irache garreta
- Fernando Pineda de la losa (vocal)
- María teresa Audicana berasategui (vocal)
- pedro Manuel Gamboa setién (vocal)