Tesis doctoral de Zafira Castaño Corsino
Role of alphacp-4, a putative tumor suppressor gene,in lung cáncer. Zafira castaño corsino faculty of science university of navarra, 2006 acp-4 is an rna binding protein coded by a gene at 3p21. It has been proposed that acp-4 may function as a «lung tumor suppressor gene. Lack of acp-4 expression is freqí¼ent in highly proliferative lung tumors. This activity is not observed in acp-4a, a splice variant whose expression is apparently normal in lung tumors. The aims of this study were to evalúate the relationship between acp-4 expression and survival of patients affected with lung cáncer, study the induction of acp-4 expression by p53, evalúate the efrect of acp-4 and acp-4a in the tumorigenic capacity of lung cáncer cells and study the proliferation status of h1299 lung cáncer cell unes when they are treated with chemotherapic drugs and acp-4 is expressed. No relationship was found between acp-4 expression and survival. By inducing damages in the dna we could see that acp-4 expression was induced by r>53. Employing transient transfection in different lung cáncer cell lines as well as infection with recombinant retroviruses in h1299 cells acp-4 and acp-4a expression was induced. A different subcellular localization of acp-4 and acp-4a was observed that could account for their different effects on cell proliferation, cell cycle arrest in g2/m, suppression of anchorage-independent growth in soft agar and reduction of the invasión capacity. Finally, tumorigenicity of h1299 cells in nude mi ce was greatly inhibited by expression of acp-4. In conclusión, expression of acp-4 is induced by p53 and can inhibit proliferation and tumorigénesis of h1299 cells by inducing cell cycle arrest. These results suggest that acp-4 inactivation may be an important event in the early stages of lung carcinogenesis.
Datos académicos de la tesis doctoral «Funcion de alfacp4 en la carcinogenesis pulmonar evaluacion de su posible papel como gen supresor de tumores«
- Título de la tesis: Funcion de alfacp4 en la carcinogenesis pulmonar evaluacion de su posible papel como gen supresor de tumores
- Autor: Zafira Castaño Corsino
- Universidad: Navarra
- Fecha de lectura de la tesis: 05/05/2006
Dirección y tribunal
- Director de la tesis
- Rubén Pio Osés
- Tribunal
- Presidente del tribunal: xose ramon Garcia bustero
- Miguel angel Molina (vocal)
- montserrat Sánchez céspedes (vocal)
- Francisco Javier Sáez castresana (vocal)