Tesis doctoral de Eduardo Rodenas Martinez
Nuclear pore complexes consist of several subcomplexes. The nup107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms and the roles of individual complex members remain elusive. We report the first description of a genetic disruption of nup107 in a metazoan. Caenorhabditis elegans nup107/npp-5 mutants display temperature-dependent lethality. Surprisingly, npp-5 is dispensable for incorporation of most nucleoporins into nuclear pores and for nuclear protein import. In contrast, npp-5 is essential for proper kinetochore localization of nup133/npp-15, another nup107 complex member, whereas recruitment of nup96/npp-10c and elys/mel-28 is npp-5 independent. We found that kinetochore protein nuf2/him-10 and aurora b/air-2 kinase are less abundant on mitotic chromatin upon npp-5 depletion. Npp-5 mutants are hypersensitive to anoxia, suggesting that the spindle assembly checkpoint (sac) is compromised. Indeed, npp-5 interacts genetically and physically with sac protein mad1/mdf-1, whose nuclear envelope accumulation requires npp-5. Thus our results strengthen the emerging connection between nuclear pore proteins and chromosome segregation.
Datos académicos de la tesis doctoral «Dissecting the roles of nup35 and nup107 in nuclear pore complex assembly and mitosis in caenorhabditis elegans«
- Título de la tesis: Dissecting the roles of nup35 and nup107 in nuclear pore complex assembly and mitosis in caenorhabditis elegans
- Autor: Eduardo Rodenas Martinez
- Universidad: Pablo de olavide
- Fecha de lectura de la tesis: 18/07/2011
Dirección y tribunal
- Director de la tesis
- Peter Askjaer
- Tribunal
- Presidente del tribunal: andrés Aguilera lópez
- Antonio Miranda vizuete (vocal)
- vincent Galy (vocal)
- tatiana García muse (vocal)