Tesis doctoral de Ainhoa Irure Yoldi
The present phd thesis is focused on the development of paramagnetic gd-based gold glyconanoparticles (gd-gnps), i.E. Nanoparticles that present on the same nanometric gold clusters both sugar conjugates and gd(iii) chelates. This work is the continuation of a research line which was started in our group with another phd thesis (d. Alcántara parra) and which was mainly dedicated to the one-pot preparation of gd-gnps as new paramagnetic probes for imaging of glioma in mice by magnetic resonance imaging (mri). The amount of gd(iii) in the gnps synthesized by this method was not under complete control. In order to solve this problem, a new protocol based on a two-steps synthesis was developed. The preparation / characterization of gd-gnps and the comparison of their relaxivity properties with contrast agents in clinical use will be presented together with the use of these gd-gnps as contrast agents for the selective labelling of cell surface receptors by mri. These biofuncional nanomaterials are water soluble, biocompatible and non-toxic to cellular lines.Finally, the application of gd-gnps as probes for in vivo mri of glioma in mice was performed in collaboration with the ¿grup d¿aplicacions biomédiques de la rmn (gabrmn)¿ at universitat autí²noma de barcelona (uab). Biodistribution studies and toxicological profile were also determined in collaboration with the group cibbim-nanomedicine at the vall d¿hebron hospital (uab).
Datos académicos de la tesis doctoral «Paramagnetic gd-based gold glyconanoparticles as probes for magnetic resonance imaging«
- Título de la tesis: Paramagnetic gd-based gold glyconanoparticles as probes for magnetic resonance imaging
- Autor: Ainhoa Irure Yoldi
- Universidad: País vasco/euskal herriko unibertsitatea
- Fecha de lectura de la tesis: 06/03/2012
Dirección y tribunal
- Director de la tesis
- Marradi
- Tribunal
- Presidente del tribunal: Fernando pedro Cossio mora
- ibane Abasolo olaortua (vocal)
- carles Arús caraltó (vocal)
- Luis Manuel Liz marzan (vocal)