Tesis doctoral de Montserrat R. Charles-harris Ferrer
The field of tissue engineering has developed in response to the shortcomings associated to the replacement of tissues lost to disease or trauma: donor tissue rejection, chronic inflammation, and donor tissue shortages. The driving force behind tissue engineering is to avoid these problems by creating biological substitutes capable of replacing the damaged tissue. This is done by combining scaffolds, cells and signals in order to create living, physiological, three-dimensional tissues. Scaffolds are porous biodegradable structures that are meant to be colonised by cells and degrade in time with tissue generation. Scaffold design and development is mainly an engineering challenge, and is the goal of this phd thesis. the main aim of this thesis is to develop and characterise scaffolds for tissue engineering applications. Specifically, its objectives are: 1. To study, optimise and characterise two scaffold processing methods: solvent casting and phase separation. This is done by experiment design analysis. 2. To characterise the degradation, surface properties, and cellular behaviour of the scaffolds produced. the scaffolds are made of a composite of polylactic acid polymer and a calcium phosphate soluble glass. The comparison of the two processing methods reveals that in general, the solvent cast scaffolds have higher porosities and lower mechanical properties than the phase-separated ones. Two compositions containing 20 weight % and 50 weight % of glass particles were chosen for further characterisations including degradation, surface properties and cellular behaviour. the degradation of the scaffolds was studied for a period of 10 weeks. The evolution of various parameters such as: morphology, weight loss, mechanical properties, thermal transitions and porosity, was monitored. Scaffolds produced via solvent casting were found to be more severely affected by degradation than phase-separated ones. the surface properties of the scaffolds were measured by modell
Datos académicos de la tesis doctoral «Development and characterisation of completely degradable composite tissue engineering scaffolds«
- Título de la tesis: Development and characterisation of completely degradable composite tissue engineering scaffolds
- Autor: Montserrat R. Charles-harris Ferrer
- Universidad: Politécnica de catalunya
- Fecha de lectura de la tesis: 17/10/2007
Dirección y tribunal
- Director de la tesis
- Josep Antoni Planell Estany
- Tribunal
- Presidente del tribunal: María pau Ginebra molins
- luigi Ambrosio (vocal)
- amedee joí«lle Vilamitjana (vocal)
- matteo Santin (vocal)