Tesis doctoral de Ana María Sanchez Gomez
With this thesis, we hope to advance our knowledge of the crocetin esters, picrocrocin and flavonoids found in the aqueous extracts of saffron spice. to do this we have worked in different areas: 1) their isolation, identification and quantification; 2) the kinetics of crocetin esters and picrocrocin, as affected by temperature; 3) the development of methods to control the quality of saffron at a commercial level; and 4) the first steps of future work lines in these compounds, such as sensory analysis and the application of ultrafiltration to the aqueous extracts of saffron. the studies carried out have been presented in seven papers in international scientific journals. in the first article, we tentatively identified crocetin esters, picrocrocin and its related compounds in the extracts from saffron and gardenia. in the second article, we tentatively identified the flavonoids in the aqueous extracts of saffron and compared the contents in samples from different geographical areas, observing that they could be differentiated by the kaempferol-3-o-sophoroside content. the third article describes the degradation kinetics of individual crocetin esters in aqueous extracts upon thermal treatment in the dark, referring to the changes in the ultraviolet-visible (uv-vis) spectra and chromatograms of the extracts, and their kinetic and thermodynamic parameters. the same kinetic studies were applied to picrocrocin in the fourth article and its taste detection threshold was studied. in the fifth article, we generated multivariate models for the rapid determination of crocetin esters and picrocrocin of saffron spice by uv-vis spectrophotometry of aqueous extracts. in the sixth article, we proposed and validated internally a solid phase extraction method for determining the picrocrocin content to be applied to quality control in commercial companies. lastly, the seventh article dealt with several centrifugal ultrafiltration devices and their performance with regard to the principal components of the aqueous extracts of saffron.
Datos académicos de la tesis doctoral «Avances en el estudio de los esteres de crocetina, picrocrocina y flavonoides del azafran especia«
- Título de la tesis: Avances en el estudio de los esteres de crocetina, picrocrocina y flavonoides del azafran especia
- Autor: Ana María Sanchez Gomez
- Universidad: Castilla-la mancha
- Fecha de lectura de la tesis: 17/02/2009
Dirección y tribunal
- Director de la tesis
- marta Alonso Diaz
- Tribunal
- Presidente del tribunal: ramón Varón castellanos
- peter Winterhalter (vocal)
- María isabel Minguez mosquera (vocal)
- petros Tarantilis (vocal)