Tesis doctoral de Farina Massimo
Metals are ubiquitous and increasing accumulation in the environment raises serious concerns in terms of environmental, occupational and consumer safety. Among other potential health effects, metal-induced carcinogenicity and neurotoxicity represent a major risk to the human society and therefore deserves to be fully assessed. In this context, a comprehensive in vitro research project in these areas needs to be conducted on selected metal compounds. the work concern in vitro metallomics investigations concerning metabolic pathways of chemically-characterized as, cd, cr, pt, mn and v compounds by experimental models developed at ecvam, such as mouse fibroblast (balb/3t3), rat pheochromocytoma (pc12) cell lines and rat brain re-aggregates and their individual cell types. The studies will be carried out by using nuclear, radioanalytical (neutron activation analysis (naa) and use of radiotracers with high specific radioactivity) and advanced spectrochemical (hplc-icpma and gfaas) techniques. They will include metal speciation concerning behaviour of test metal compounds in cell culture media, uptake, intracellular distribution, binding with cell components. steps of the research project: (i) preparation of radiotracers for labelling of different concentrations of metal compounds (ii) exposure of standardised in vitro models to stable and/or radiolabelled metal compounds for different time periods and range of concentration (iii) mechanistic studies involving the establishment of dose-effect relationship in relation to uptake of metal tested by whole cell compartments as obtained by differential centrifugation as well as gel filtration techniques. Determination of the oxidation state of metallome in cell compartments (iv) statistical treatment of the data and development of metabolic models.
Datos académicos de la tesis doctoral «Metallomics in in vitro toxicology research«
- Título de la tesis: Metallomics in in vitro toxicology research
- Autor: Farina Massimo
- Universidad: Autónoma de barcelona
- Fecha de lectura de la tesis: 24/11/2008
Dirección y tribunal
- Director de la tesis
- Enrico Sabbioni
- Tribunal
- Presidente del tribunal: amadeu Creus capdevila
- salvador Fortaner torrent (vocal)
- (vocal)
- (vocal)