Tesis doctoral de Kathrin Pallauf
Macroautophagy refers to bulk degradation of cytoplasmic contents by sequestration in a vesicle called autophagosome which fuses with the lysosome. After degradation the molecules obtained can be reused. Macroautophagy works as a starvation response und thus is regulated by the ser/thr kinase tor (target of rapamycin). The formation of the autophagosome is mediated by the autophagy related proteins, the atgs. Atg5 and atg7 are part of the ubiquitin-like systems that promote elongation of the autophagosome membrane by lipidating another atg atg8/lc3. excessive macroautophagy can lead to cell death which made it possible to identify an autophagy inducer in our laboratory by screening a cdna library for cell death inducers. As the protein encoded for by the identified cdna has a structure similar to many transmembrane receptors, we named this protein autophagy inducing receptor (air). in this thesis a functional and structural study of this protein is carried out. It is found to be a transmembrane protein type i that transiently localises to the cell membrane but predominantly localises to the endosomal-lysosomal compartments. The over-expressed protein induces autophagy in various human cell lines. The functional domain for this property was found to be the intracellular region, as the intracellular domain is necessary and sufficient to induce autophagy. Within this region the autophagy inducing properties could be ascribed to a 19 amino acid region. The pathway induced by this protein depends on the core autophagy related proteins atg5 and atg7.
Datos académicos de la tesis doctoral «Functional and structural study of a novel transmembrane protein and its role in autophagy«
- Título de la tesis: Functional and structural study of a novel transmembrane protein and its role in autophagy
- Autor: Kathrin Pallauf
- Universidad: Salamanca
- Fecha de lectura de la tesis: 08/07/2011
Dirección y tribunal
- Director de la tesis
- Felipe Pimentel Muiños
- Tribunal
- Presidente del tribunal: faustino Mollinedo García
- ines María Anton gutierrez (vocal)
- ignacio Moreno de alborán (vocal)
- María José Caloca roldan (vocal)