Tesis doctoral de Angela Cánovas Tienda
This phd is part of a line of research devoted to studying the genetic basis of lipid metabolism and fat deposition in pigs with a view to producing healthy and high quality meat. The main objective is the identification of polymorphisms and regulatory mechanisms responsible for the genetic variability of these complex characters in pigs. In this sense, we have used several methods in the fields of structural (expression qtl (eqtl) maps; candidate genes studies) and functional (gene expression studies) genomics and also protein and cell studies in muscle and fat samples from pigs selected by meat quality parameters. In this context, using microarrays we analyzed the mrna expression pattern in gluteus medius muscle samples obtained from a commercial duroc pig population with divergent phenotypes for several parameters related to lipid deposition. As a result, we have obtained a list of genes differentially expressed between animals with divergent profiles related to lipid deposition. The ontological/functional study showed that these genes were particularly related to lipid metabolism, growth and muscle differentiation, immunity and glucose uptake in the insulin pathway. Moreover, analysis of eqtl has revealed the existence of genomic regions responsible for the variation of gene expression in porcine gluteus medius muscle. Some of these eqtl show positional concordance with several qtl related to meat quality and fat deposition previously identified in the same duroc population. Additionally, we have performed a comprehensive study of four candidate genes (acaca, hmgcr, scd and ¿6d) directly involved in traits related to meat quality, playing an important role in fatty acids and cholesterol synthesis and desaturation. Combining the results of gene expression analysis, eqtl maps and candidate genes studied have resulted in a list of functional and positional candidate genes representing a valuable contribution to the understanding of the genetic regulation of skeletal muscle individual gene expression in swine species. This is a first step towards disentangling gene networks and molecular mechanisms involved in muscular lipid metabolism and meat quality traits in pigs.
Datos académicos de la tesis doctoral «Genes asociados con la deposición y composición de grasas en porcino: estudios de expresión génica, proteínas y genética funcional y estructural«
- Título de la tesis: Genes asociados con la deposición y composición de grasas en porcino: estudios de expresión génica, proteínas y genética funcional y estructural
- Autor: Angela Cánovas Tienda
- Universidad: Lleida
- Fecha de lectura de la tesis: 08/03/2011
Dirección y tribunal
- Director de la tesis
- Ramona Natacha Pena Subirá
- Tribunal
- Presidente del tribunal: joan Estany illa
- josep María Folch albareda (vocal)
- Ana isabel Fernandez avila (vocal)
- Juan José Arranz santos (vocal)