Contribution of antioxidant enzymes to toxicity assessment in fluvial biofilms

Tesis doctoral de Chloé Bonnineau

To evaluate the impact of contamination on aquatic ecosystem, this thesis focused on a multi-biomarker approach, including molecular biomarkers, in biofilms. To complete the traditional tool-box of biofilm biomarkers, antioxidant enzymes activities (aea) of catalase, ascorbate peroxidase and glutathione reductase were shown to be biomarkers of oxidative stress in biofilms. Though aea can be influenced in the same extent by natural factors (biofilm age, colonization or exposure light) and toxicants (herbicides and pharmaceuticals), they provided valuable information to understand the chemicals effects. In particular, short-term toxicity tests are of interest to compare communities capacity to cope with oxidative stress and to know their exposure history to such stress. This multi-biomarker approach at community-level was found to be especially interesting to assess toxicity of emerging pollutants (íY-blockers) on non-target communities. To improve this approach, the feasibility of measuring gene expression in biofilms using a functional gene array was also shown.

 

Datos académicos de la tesis doctoral «Contribution of antioxidant enzymes to toxicity assessment in fluvial biofilms«

  • Título de la tesis:  Contribution of antioxidant enzymes to toxicity assessment in fluvial biofilms
  • Autor:  Chloé Bonnineau
  • Universidad:  Girona
  • Fecha de lectura de la tesis:  23/06/2011

 

Dirección y tribunal

  • Director de la tesis
    • Helena Guasch Padró
  • Tribunal
    • Presidente del tribunal: helmut Segner
    • martin Laviale (vocal)
    • etelvina María De almeida paula figueira (vocal)
    • pilar Mateo ortega (vocal)

 

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