Tesis doctoral de Medya Shikhagaie
We have investigated the previously uncharacterized human cytomegalovirus (hcmv) ul1 open reading frame (orf), a member of the lately evolving hcmv rl11 gene family. Ul1 is hcmv specific; absence of ul1 in chimpanzee cmv (ccmv) and sequence analysis studies suggests that ul1 may have originated by duplication of an ancestor gene from the rl11-trl-cluster (trl11, trl12 and trl13). sequence similarity searches against human immunoglobulin (ig) containing proteins revealed that hcmv pul1 shows significant similarity to the cellular carcinoembrionic antigen related (cea) protein family n- terminal ig domain, which is responsible for cea ligand recognition. Northern blot analysis revealed that ul1 is transcribed during the late phase of the hcmv replication cycle, both in fibroblast-adapted and endotheliotropic strains of hcmv. We characterized the protein encoded by an ha-tagged ul1 in the ad169-derived hb5 background. Ul1 is expressed as a 224 amino acid type i transmembrane glycoprotein which becomes detectable not earlier than 48 h to 72 h post infection. In infected human fibroblasts, pul1 co-localized at the cytoplasmic site of virion assembly and secondary envelopment together with tgn-46, a marker for the trans-golgi network, and viral structural proteins including envelope glycoprotein b and tegument phosphoprotein pp28. Furthermore, analysis of highly purified ad169 ul1-ha epitope tagged virions revealed that pul1 is a novel constituent of the hcmv envelope. Importantly, deletion of ul1 in hcmv tb40/e resulted in a reduced growth in a cell type-specific manner, suggesting that pul1 may be implicated in regulating hcmv cell tropism. palabras clave de la tesis: cytomegalovirus, virion, cell tropism, glycoprotein, gene expression
Datos académicos de la tesis doctoral «Characterization of ul1, a member of the human cytomegalovirus rl11 gene family«
- Título de la tesis: Characterization of ul1, a member of the human cytomegalovirus rl11 gene family
- Autor: Medya Shikhagaie
- Universidad: Pompeu fabra
- Fecha de lectura de la tesis: 07/11/2011
Dirección y tribunal
- Director de la tesis
- Miguel Lopez Botet Arbona
- Tribunal
- Presidente del tribunal: pablo Engel rocamora
- Alberto Fraile ramos (vocal)
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- (vocal)